The production of active yeast, as starting material for producing various biotechnology products (i.e. beta-glucan, yeast extract, yeast cell wal l), is accomplished using aerobic fermentors through the yeast culture processes.
Yeast culture processes can divided into 3 parts. First, Yeast laboratory process, yeast is cultivated in a small scale laboratory apparatus ( test tube and flask ) with a specific culture media. In the second step, Yeast seed process, yeast seed production is scaled up from yeast inoculum through various units of seed fermentors until to F1-unit production fermentor. During this stage, molasses (as carbon source) and defoamer agent are added. After the effective concentration of yeast is achieved, the growth-broth is centrifuged to form a yeast cream (18-20% solid). At the latest step,yeast cream is mixed with nutrient and then transferred to 30 tons fermentor to begin the fermentation process under a specific set of conditions. Once the optimum condition is reached, the entire fermented media is filtered through a highly-efficient filter press to make yeast cream (30% solid).
The active yeast product is obtained after crushing, packaging, and finally stored in a cold room.
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